Development and characterization of murine monoclonal antibody specific for the P1.4 PorA proteins from strain B:4:P1.(7b).4. of Neisseria meningitidis

  • María Elena Pérez Instituto Finlay
  • Ramón Barberá Instituto Finlay
  • Francisco Domínguez Instituto Finlay
  • Oscar Otero Instituto Finlay
  • Mercedes Gutiérrez Instituto Finlay
  • Gustavo Falero Instituto Finlay
  • Grethel Reyes Instituto Finlay
  • Franklin Sotolongo Instituto Finlay
  • Gustavo Sierra Instituto Finlay
Keywords: monoclonal antibodies, PorA, Neisseria meningitidis

Abstract

Neisseria meningitidis isolates are conventionally classified by serosubtyping that characterizes the reactivities of the PorA outer membrane protein variable-region epitopes with monoclonal antibodies. Porins are outer membrane proteins (OMPs) of N. meningitidis serogroup B and have attracted study principally for two reasons: their use in the classification of meningococcal isolates into serotype and subtype and as potential components of vaccines against this important pathogen. New murine hybridomas, secreting specific monoclonal antibodies against PorA serotype P1.4 of N. meningitidis serogroup B, were generated using conventional hybridoma procedures. The monoclonal antibodies obtained were characterized by Western blot and whole cell ELISA, using reference strains from different N. meningitidis serotypes and subtypes. All monoclonal antibodies belong to isotype IgG1. Others hybridomas producing MAbs against PorB and FrpB were also obtained.

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Published
2016-03-15
How to Cite
Pérez, M., Barberá, R., Domínguez, F., Otero, O., Gutiérrez, M., Falero, G., Reyes, G., Sotolongo, F., & Sierra, G. (2016). Development and characterization of murine monoclonal antibody specific for the P1.4 PorA proteins from strain B:4:P1.(7b).4. of Neisseria meningitidis. VacciMonitor, 20(2), 6-10. Retrieved from https://vaccimonitor.finlay.edu.cu/index.php/vaccimonitor/article/view/110
Issue
Vol 20 No 2 (2011): Mayo.-ago.
Section
Original papers

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