Resonancia magnética nuclear: nuevas aplicaciones en la cuantificación y la evaluación de intermediarios de vacunas basadas en polisacáridos
Resumen
La resonancia magnética nuclear (RMN) constituye una fuerte alternativa para estudios estructurales, evaluación de identidad y cuantificación de ingredientes farmacéuticos activos (IFA). En las dos últimas décadas la aplicación de la resonancia magnética nuclear cuantitativa (RMNc) tuvo un creciente impacto en la cuantificación de compuestos, fundamentalmente orgánicos. Varios resultados obtenidos mediante la RMNc han ido creando un lugar para la técnica en la industria biofarmacéutica. La RMNc incluye experimentos de RMN con algunos parámetros modificados, con el objetivo de obtener señales cuantificables. Basada en algunos de los reportes más relevantes, la presente revisión aborda algunas de las aplicaciones de la RMN para vacunas basadas en polisacáridos o glicoproteínas. El trabajo hace especial énfasis en detallar algunos aspectos que caracterizan la RMNc, así como sus aplicaciones.Citas
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