Estrategias de obtención de proteínas recombinantes en Escherichia coli

  • José García Centro de Ingeniería Genética y Biotecnología
  • Zeila Santan Centro de Ingeniería Genética y Biotecnología
  • Lourdes Zumalacárregui Facultad de Ingeniería Química Instituto Superior Politécnico "José Antonio Echeverría"
  • Marisel Quintana-Esquivel Centro de Ingeniería Genética y Biotecnología
  • Diamilé González Centro de Ingeniería Genética y Biotecnología
  • Gustavo Furrazola Centro de Ingeniería Genética y Biotecnología
  • Oscar Cruz Centro de Ingeniería Genética y Biotecnología
Palabras clave: proteína recombinante, Escherichia coli, factores genéticos, factores fisiológicos, fermentación

Resumen

La expresión de proteínas recombinantes se ha favorecido con el uso de Escherichia coli debido a su relativo bajo costo, alta densidad de cultivo, su fácil manipulación genética y a las diversas herramientas biotecnológicas disponibles que son compatibles. En este artículo se presentan algunas estrategias para la expresión de Escherichia coli; se destacan factores genéticos y fisiológicos que incluyen: número de copias del vector de expresión, características del gen, estabilidad del ácido ribonucleico mensajero, promotor empleado, cepa utilizada, composición del medio de cultivo, parámetros de operación en el fermentador y también se abordan la conservación de cepas y la estrategia de cultivo y purificación.

Citas

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Publicado
2015-12-08
Cómo citar
García, J., Santan, Z., Zumalacárregui, L., Quintana-Esquivel, M., González, D., Furrazola, G., & Cruz, O. (2015). Estrategias de obtención de proteínas recombinantes en Escherichia coli. VacciMonitor, 22(2), 30-39. Recuperado a partir de http://vaccimonitor.finlay.edu.cu/index.php/vaccimonitor/article/view/51
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